Metabolomics Facility LeidenMetabolomics Facility Leiden

Contact details:

Prof. dr. Thomas Hankemeier
Einsteinweg 55 2333 CC Leiden

The Metabolomics Facility Leiden offers a highly structured environment for advanced metabolomics studies. The facility offers fully validated, state-of-the-art platforms that each cover a part of metabolism and together span the complete human metabolome. In addition, expertise is available to support screening projects and identifying active compounds in natural products. The facility operates at the frontier of metabolomics developments, starting with involvement in experimental design and sample collection to the actual measurements, data analysis, identification of unknown compounds and comprehensive feedback on the results to ensure the best fit between biological question and optimal use of our highly advanced facilities.

The Metabolomics Facility Leiden consists of two entities: (1) the BioMedical Metabolomics
Facility Leiden (BMFL) and (2) the Natural Products Lab (NPL). Both operate as one facility
under one management and are located in the Faculty of Science of Leiden University. The
Metabolomics Facility Leiden is a core facility of Leiden University and works closely together
with LUMC, Erasmus MC, other Dutch and international academic and clinical research
groups, and pharma, food and biotech companies. The facility works together with expertise
groups in the Leiden Academic Centre for Drug Research (LACDR: Systems Pharmacology,
Biopharmaceutics, Drug Delivery Technology), the Leiden Institute of Advanced Computer
Science, the Leiden Centre for Data Science, and the Institute of Biology Leiden (IBL:
Microbial Biotechnology, Cell Biology).
BMFL offers a highly structured environment for advanced metabolomics studies. BMFL
builds on fully validated, state-of-the-art platforms that each cover a part of the human
metabolism and together span the complete human metabolome. The metabolomics
platforms are based on gas chromatography, liquid chromatography and capillary
electrophoresis coupled with mass spectrometry (GC-MS, LC-MS and CE-MS). BMFL is
particularly geared towards biomarker discovery of human disease and health.
BMFL works according to ISO17025 guidelines and employs rigorous quality standards,
working with well validated methods and using an optimized quality control pipeline which
provides correction for analytical variation, and data monitoring ensuring robust data that
enable truly comparative studies.
BMFL is the core facility of the Netherlands Metabolomics Centre (NMC), a national publicprivate
consortium that had a research program of about 50 M€ in the period 2008-2013.
BMFL’s users include internal as well as external academic and clinical collaborators, and
pharmaceutical and industrial clients from all over the world, measuring over 15,000 profiles
annually. In close collaboration with partners, BMFL defines a tailor-made approach that
offers the best fit between the collaborator’s biological question and optimal use of our
highly advanced facilities.
This facility consists of AB Sciex 5600 Triple-ToF, Agilent 6460 Triple-quadrupole-MS, Agilent
6490 Triple-quadrupole-MS, Agilent CE-ToF-MS, Tecan Freedom Evo, Agilent GC-MS, Agilent
HPLC-chip G4240A, Shimadzu LCMS-8050 Triple-quadrupole MS, Thermo Scientific LTQIontrap,
Thermo Scientific LTQ-FT-ICR-MS, Thermo Scientific LTQ-Orbitrap-MS, Bruker
Maxis Q-ToF-MS, Advion NanoMate, Agilent 6530 Q-ToF-MS, Waters SYNAPT G2-s, and
Waters Xevo TQ-S.
The Natural Products Lab (NPL), located within the Institute of Biology Leiden (IBL) of
Leiden University, is a unique facility in the Netherlands, focusing on natural products
research, traditional medicine, ethnobotany and phytochemistry. It brings in expertise and
facilities in the following areas:
1) Extraction and isolation of target compounds from natural products at both an analytical
and preparative scale; chromatographic or spectroscopic-based phytochemical analytical tools such as LCMS, NMR techniques, GCMS, SFC, etc.;
3) Identification and/or structural elucidation of isolated compounds;
4) Chemical fingerprinting using metabolomics or metabolic profiling techniques;
5) Scaling-up of developed methods to production scale.
Instruments available at the NPL include:
Büchi E-916, Bruker GC-ACPI-Qtof, Agilent GC/FID: Agilent 6890, Agilent 7890 GCMS
(single quad), Agilent 1200 HPLC/DAD, Agilent 1200 HPLC/DAD/ELSD, Büchi C605
Sepacore, Agilent 1260 SFC/DAD/MS, Büchi Syncore and Bruker UHPLC-Q-ToF-MS. NMR
access is provided through the Leiden NMR facility.

Connection to strategic developments
Life Sciences & Health
Health and Food